Following the success using the Orbitrap Astral for rapid protein analysis, we have been exploring it’s use for deep and rapid PTM mapping. In an article published in Nature Communications, we describe the use of a data-independent acquisition (DIA) approach to identify over 29,000 phosphorylation sites within 30 minutes. We applied this rapid phosphoproteomic method to generate a comprehensive mouse phosphoproteome atlas, detecting 81,120 unique phosphorylation sites in 12 hours across 12 tissues. We have continued to expand our PTM efforts to include glycopeptide analysis. In 30 min of analysis time we can identify over 4,000 unique glycopeptides from a sample of glycosylation-enriched mouse brain.
Fast and deep phosphoproteome analysis with the Orbitrap Astral mass spectrometer