The NCQBCS is outfitted with several state-of-the-art mass spectrometry systems. These instruments are used on the various ongoing technology development projects. Improvements made on these devices are then easily disseminated to current commercial instruments. Further, collaborator projects are also conducted on the very same equipment ensuring the best results possible.
Instruments we use in our lab
Orbitrap Astral: Latest generation Orbitrap with Astral mass analyzer for high throughput, deep coverage, high sensitivity, and accurate quantitation. (2 systems)
Orbitrap Ascend Tribrid: Latest generation, ETD and PTR-enabled quadrupole-Orbitrap-dual cell quadrupole linear ion trap hybrid system (Qq-OT-QLT). (2 systems)
Orbitrap Exploris 240: Quadrupole Orbitrap MS system for high data quality and depth of analysis.
Q Exactive UHMR: Ultra-High Mass Range (UHMR) quadrupole-Orbitrap hybrid system (Qq-OT) for native intact mass and top-down analysis. (2 systems)
LTQ Velos Dual Linear Ion Trap: ETD-enabled, dual-pressure linear ion trap mass spectrometer that has been modified to perform NETD and AI-ETD.
Q Exactive Focus Hybrid Quadrupole-Orbitrap: High-resolution accurate-mass quadrupole-Orbitrap hybrid system (Qq-OT) with restricted TopN capabilities.
ISQ Single Quadrupole GC/MS system: Single quadrupole system with Trace 1310 gas chromatograph and TriPlus RSH autosampler for robotic sample handling.
Modified LTQ Mass Spectrometer: Custom quadrupole linear ion trap system with a modified ETD reagent source.
Vanquish Neo UHPLC: All-in-one nano-, capillary-, and micro-flow LC for high-sensitivity LCMS workflows and low-flow applications. (5 systems)
Vanquish UHPLC: Latest generation binary pump (up to 1,500 MPa) equipped with split HT autosampler and column heater. (2 systems)
Dionex UltiMate 3000 nano UHPLC: Equipped with a RS autosampler. (2 systems)
Dionex UltiMate 3000 UHPLC: Normal-flow ultra-high-pressure liquid chromatography pump equipped with UV detectors, column heaters, and RS autosampler. One system is connected to a fraction collector. (5 systems)
TriVersa NanoMate (Advion): Automated ion source platform for high throughput infusion.
1260 Infinity II HPLC (Agilent Technologies): Normal-flow ultra-high-pressure liquid chromatography pump equipped with a column heater, a UV detector, and an automated fraction collector. Offline for preparative work.
Zip Chip (908 Devices): Autosampler for on-chip SPE proteomics and metabolomics analysis using a complete workflow.
Proteograph System (Seer): Automated plasma sample preparation system for mass spectrometry workflow.
STARlet (Hamilton): Programmable automated liquid handling system.
Fractionator (Basi): Refrigerated dual channel fraction collector.
Ultra-high-pressure column packing station (In-house made): Reinforced glass enclosure that houses ultra-high-pressure pump and fittings (up to 50,000 psi) connected to a packing slurry chamber and equipped with a microscopic camera.
P-2000 and P-1000 Micropipette Puller (Sutter Instruments): for fabrication of micropipettes, optical fiber probes, and nanospray tips.
Plasma Cleaner PDC-001-HP & PlasmaFlo PDC-FMG (Harrick): with supplemental gas mixing for contactless cleaning of sensitive surfaces and hydrophilic coating.
Tormach 1100M 4-axis CNC Mill: equipped with automatic tool changer, used for building custom parts and equipment.
Tormach PCNC 440 Benchtop Mill: designed for fabrication of small instrument components.
Tormach 15L Slant-PRO CNC Lathe: equipped with automatic collet closer and turret
Bambu Lab X1: CoreXY printer
For additional information about NCQBCS please send your contact details (name, institution, email) along with a short description of how we can help to laura[dot]vantoll[at]wisc[dot]edu.
Note, if you are interested in training, please use the form on our training page.
Coon Lab, University of Wisconsin-Madison, 440 Henry Mall, Madison, WI
Li Lab, University of Wisconsin-Madison, 777 Highland Avenue, Madison, WI