Lipidomics Workshop Schedule
All sessions will be held via Zoom
All times are in CST time zone (Chicago)
Schedule subject to change
Monday, March 20
Data Acquisition
12:30-1:45 – Chromatography and MS Acquisition (Lecture)
Topics include: Hilic, mobile phase, reverse phase, stationary phase, fragmentation, DDA; polarity +/-, activation, RTLS
Yuchen He and Dain Brademan – Coon Lab at the University of Wisconsin
1:45-2:45 – Method Setup on QExactive, Tribrid and Exploris (Demo)
Dain Brademan – Coon Lab at the University of Wisconsin
2:45-3:00 – Break
3:00-4:00 – Research Lecture
Alan Saghatelian – Salk Institute for Biological Sciences
4:00-5:00 – Q&A and Plans for Tomorrow (Discussion)
Katie Overmyer – Coon Lab at the University of Wisconsin
Tuesday, March 21
Data Processing
9:00-10:00 – Research Lecture (Lecture)
Judi Simcox – Assistant Professor, University of Wisconsin-Madison
10:00-11:00 – Lipid Overview (Lecture)
Topics include: peak integration, identification based on fragmentation, pairing data, accounting for junk
Paul Hutchins – Dow Scientist and creator of Lipidex
11:00-12:00 – Lipidex & Compound Discover (Demo)
Topics include: Lipid classes, how to setup a run, spectral search; peak finder, MSn searching
Ben Anderson – Coon Lab at the University of Wisconsin
12:00-1:00 – Break
1:00-2:15 – Quality Control (Lecture)
Topics include: Batch effect, sample pooling, reproducibility, internal, RSDs, linear dynamic range, RT vs M/Z, choosing parameters, manual validation, spectral Interpretation
Yuchen He and Ben Anderson – Coon Lab at the University of Wisconsin
2:15-2:30 – Break
2:30-3:30 – Data Analysis Tools (Lecture)
Katie Overmyer – Coon Lab at the University of Wisconsin
3:30-4:30 – Library Building (Demo)
Trent Peters-Clarke and Dain Brademan – Coon Lab at the University of Wisconsin
4:30-5:00 – Q&A (Discussion)
Katie Overmyer – Coon Lab at the University of Wisconsin