Lipidomics Masthead

 

 

 

Lipidomics Workshop

 
March 20-21, 2023 via Zoom
This interactive workshop will cover the fundamentals of chromatography and data lipid acquisition on a mass spectrometer, lipid data processing and library building through a mix of lectures, demos and discussion.

This is an intermediate level workshop, and is open to graduate students, post-docs, faculty and staff who have at least 6 months of mass spec hands-on use and/or experience processing mass spec data.

This event is made possible by generous funding from the National Institutes of Health National Center for Quantitative Biology of Complex Systems (2P41GM108538).

 

Lipidomics Schedule


Lipidomics Workshop Schedule

All sessions will be held via Zoom
All times are in CST time zone (Chicago)
Schedule subject to change


Monday, March 20
Data Acquisition

12:30-1:30 – Research Lecture
Alan Saghatelian – Salk Institute for Biological Sciences

1:30-2:45 – Chromatography and MS Acquisition (Lecture)
Topics include: Hilic, mobile phase, reverse phase, stationary phase, fragmentation, DDA; polarity +/-, activation, RTLS
Yuchen He and Dain Brademan – Coon Lab at the University of Wisconsin

2:45-3:00 – Break

3:00-4:00 – Method Setup on QExactive, Tribrid and Exploris (Demo)
Dain Brademan – Coon Lab at the University of Wisconsin

4:00-5:00 – Q&A and Plans for Tomorrow (Discussion)
Katie Overmyer – Coon Lab at the University of Wisconsin


Tuesday, March 21
Data Processing

9:00-10:00 – Research Lecture (Lecture)

10:00-11:00 – Lipid Overview (Lecture)
Topics include: peak integration, identification based on fragmentation, pairing data, accounting for junk
Yuchen He and Dain Brademan – Coon Lab at the University of Wisconsin

11:00-12:00 – Lipidex & Compound Discover (Demo)
Topics include: Lipid classes, how to setup a run, spectral search; peak finder, MSn searching
Ben Anderson – Coon Lab at the University of Wisconsin

12:00-1:00 – Break

1:00-2:15 – Quality Control (Lecture)
Topics include: Batch effect, sample pooling, reproducibility, internal, RSDs, linear dynamic range, RT vs M/Z, choosing parameters, manual validation, spectral Interpretation
Yuchen He and Ben Anderson – Coon Lab at the University of Wisconsin

2:15-2:30 – Break

2:30-3:30 – Data Analysis Tools (Lecture)
Katie Overmyer – Coon Lab at the University of Wisconsin

3:30-4:30 – Library Building (Demo)
Trent Peters-Clarke and Dain Brademan – Coon Lab at the University of Wisconsin

4:30-5:00 – Q&A (Discussion)
Katie Overmyer – Coon Lab at the University of Wisconsin

Lipidomics Registration


Ready for a career-changing experience?

There is no registration fee, however if you register you have made a commitment to attend.

We will be recording this workshop on Zoom, as part of the registration process you will need to consent to being recorded.

We want this workshop to be as interactive as possible, therefore you will be required to have your camera turned on during the event. We promise not to judge your messy desk and we know you will probably be sitting in a room of other people.

If these conditions are not agreeable for you, please do not register for the workshop. We will post the recordings on YouTube, so you will still have access to the material.

Registration for Lipidomics Workshop

Due to space limitations, all who apply will not be admitted.
Incomplete applications will not be considered.

If you have questions or difficulty in registering, please email Laura.

This event is made possible by generous funding from the National Institutes of Health National Center for Quantitative Biology of Complex Systems (2P41GM108538).

Lipidomics downloadable PDFS

Download Documents from 2023 Lipidomics Workshop

PROGRAM Schedule Lecture Recordings