Mass spectrometry-based quantitative lipidomics is an emerging field aimed at understanding the relationships between lipidomes and disease development. However, quantifying lipidomes comprehensively in a high-throughput manner is challenging because of the complex structure of lipids. This study shows a diazobutanone-assisted isobaric labelling strategy that can be used as a rapid and robust platform for multiplexed quantitative lipidomics across a broad range of lipid classes, including phospholipids and glycolipids.
Read the full article: Diazobutanone-assisted isobaric labelling of phospholipids and sulfated glycolipids enables multiplexed quantitative lipidomics using tandem mass spectrometry