We continue to refine the laser alignment and implementation of the IRMPD on the Orbitrap Ascend. Recently we have leveraged these improvements to better sequence modified RNA and RNA therapeutics. Our study highlighting the improved sequencing of phosphorothioate using negative electron-transfer dissociation (NETD) alongside complementary photodissociation methods (IRMPD) was recently published in Molecular and Cellular Proteomics.
Phosphorothioate RNA Analysis by NETD Tandem Mass Spectrometry