13 Feb 2017

Ultra-plexed Proteome Analysis

13 Feb 2017
 

Ultra-plexed Proteome Analysis

Presently our DiLeu tagging chemistry stands at the forefront of multiplexed labeling approaches by offering parallel analysis of up to twelve proteomes.  To achieve this level of plexing we leverage the slight mass differences in neutron binding energy between carbon, nitrogen, and hydrogen through unique isotopic formulations that differ in mass by as little as 6 mDa (just as in the NeuCode labels of TR&D #2). For twelve-plex DiLeu, twelve discrete reporter ions in the mass range of m/z 115-118 can be distinguished in MS2 scans acquired at a resolving power of 30,000. We are currently developing additional DiLeu isotopologues that differ in mass by 3 mDa to create a 21-plex set of labels and an unparalleled level of quantitative throughput.

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