HAo et al

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04 Dec 2019

Metandem, a free and online software for MS-based isobaric labeling metabolomics

By nickwiecien

Hao et al. (2019) recently published a paper in Analytica Chimica Acta detailing the utility of Metandem, a data analysis software which is aids in isobaric labeling-based metabolomics.

While mass spectrometry-based stable isotope labeling is advantageous compared to other methods of isotope labeling due to its multiplexing and accurate quantification capabilities, its data analysis requires specifically customized bioinformatic tools. However, Metandem, a free, unique and online software, can aid in the analysis of stable isotope labeling-based metabolomics data.

Metandem has a number of different features that assist in MS-based isobaric labeling, such as integrating feature extraction, metabolite quantification and identification, batch processing of multiple data files, online parameter optimization for custom datasets, data normalization and statistical analysis.

Metatandem is available free and online at http://metandem.com/web/. 

Graphical abstract for Metandem paper published by Hao et al in Analtica Chimica Acta demonstrating the software's utility in isobaric labeling, integrating feature extraction, and metabolite quantification.
02 Oct 2019

Acute-Phase proteins differ in mice with and without prostate inflammation

By nickwiecien

L. Hao et al conducted a quantitative proteomic analysis on induced prostate inflammation in mice and published a paper in the American Journal of Physiology-Renal Physiology.

Researchers compared the quantitative proteomic analysis of urine from mice with and without prostate-specific inflammation. Prostate inflammation as it is a key symptom of many different prostate conditions, such as infection and cancer, and therefore by doing so one gains a better understanding of disease mechanisms.

Researchers induced prostate-specific inflammation by conditional prostate epithelial IL-1β expression. Next, they ran urine sample tests and quantified urinary proteins. L. Hao et al found that different levels of acute-phase response proteins (proteins which have plasma concentrations that increase or decrease in response to inflammation) were represented between mice with and without prostate inflammation; these were haptoglobin, inter-α-trypsin inhibitor, and α1-antitrypsin 1-1.

Mass-spectrometry-based quantitative urinary proteomics is an important and powerful method for discovering biomarkers and uncovering molecular urological mechanisms.

Graphical abstract for Hao et al, depicting the quantitative proteomic analysis of mice urine.