Proteomic atlas of human brain in Alzheimer’s disease


Collaborating Investigator/s

Subhojit Roy (University of Wisconsin-Madison)



To reveal proteomic differences between various brain regions, especially deep characterization of tissues was required that could not be achieved using standard single-shot label-free proteomics method. The methodology used in the project for the first-time combined label-free quantification and extensive peptide fractionation demonstrating that highly reproducible quantification of over 10K proteins in human tissues could be achieved this way. This combination of techniques could be used in the future when particularly in-depth characterization of samples is desired.