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Chemical labeling for glycan and glycopeptide quantitation

Growing implications of glycosylation in human disease have prompted intensive focus on revealing glycomic perturbations through absolute and relative quantification. Empowered by an increasing capacity for detection, identification, and characterization, the past decade has provided a significant increase in the number of suitable strategies for glycan and glycopeptide quantification. In this review, Delafield and Li present the most recent advances in chemical labeling and associated techniques for glycan and glycopeptide quantification.

Read the article: Recent Advances in Analytical Approaches for Glycan and Glycopeptide Quantitation

Selecting a Labeling Strategy for Quantitative Proteomics of Multiple Samples

Buchberger AR et al (2019) recently published a chapter reviewing various labelling strategies for quantitative proteomic analysis in Mass Spectrometry-Based Chemical Proteomics.

Specifically, the chapter reviews strategies such as label-free quantitation, metabolic labeling, and chemical stable isotope labeling, and also discusses which labeling approach is best for various types of proteomic analyses. The chapter also provides an explanation on how to use N,N‐dimethyl alanine (DiAla) and N,N‐ dimethyl valine (DiVal) isobaric labeling strategies for quantitative analyses in ways which are economic and effective.

The chapter states that quantitative proteomics is crucial for biomarker discovery in studying and understanding various diseases and biological research, as proteins are crucial in all biological processes. Because biomarker studies can be time-consuming, heavily reliant on instruments and vary depending on the strategy used, selecting the appropriate labeling strategy is important in quantitative analysis.

A graphical abstract from Buchberger et al (2019) which depicts labeling strategy options for a type of sample. The sample in the picture is a mouse, the labeling strategies include 5-plex iDiLeu, 12-Plex DiLeu, 4-Plex DiLeu, 2-Plex mDiLeu, and 4-Plex DiAla.