high-mass accuracy data

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Software Highlight: Morpheus

Morpheus, a custom search algorithm designed specifically for high-resolution tandem mass spectra, is one of many free softwares offered by NCQBCS.

Developed by the Coon Lab, Morpheus works specifically with high-mass accuracy data, and is superior to other similar programs, such as those originally designed for low-resolution MS/MS. For instance, compared to Mascot, Open Mass Spectrometry Search Algorithm (OMSSA) and Sequest, Morpheus can identify more spectra, peptides and proteins at a 1% false discovery rate. Morpheus is also 1.5-4.6 times faster than OMSSA.

Morpheus is free and open source under a permissive license.

The manuscript for Morpheus is available here, and the software can be downloaded here. Additionally, information on other software that the National Center for Quantitative Biology of Complex Systems offers can be found here.

Graphical abstract for the Morpheus manual, demonstrating the algorithm's matching capabilities 
Morpheus score =10.302, 10 matching products +30.2% matching abundance.