Glycosylation

Viewing posts tagged Glycosylation

08 Sep 2021

Introducing DiLeuPMP, a multiplexed isobaric labeling method

By nickwiecien

Glycosylation plays an important role in how the human body functions, including cell recognition, signaling, and immune response. While efforts have been devoted to the analysis of N-glycans, high-throughput quantitative analysis of O-glycans is underexplored. In this study, a multiplexed isobaric labeling method, DiLeuPMP, is introduced. This method combines the release and labeling of O-glycans in one step and achieves accurate MS2-based relative quantification. This method provides an effective and reliable approach for the profiling and high-throughput quantitative analysis of O-glycans in complex samples.

Read the article: DiLeuPMP: a multiplexed isobaric labeling method for quantitative analysis of O-glycans

11 Aug 2021

Chemical labeling for glycan and glycopeptide quantitation

By nickwiecien

Growing implications of glycosylation in human disease have prompted intensive focus on revealing glycomic perturbations through absolute and relative quantification. Empowered by an increasing capacity for detection, identification, and characterization, the past decade has provided a significant increase in the number of suitable strategies for glycan and glycopeptide quantification. In this review, Delafield and Li present the most recent advances in chemical labeling and associated techniques for glycan and glycopeptide quantification.

Read the article: Recent Advances in Analytical Approaches for Glycan and Glycopeptide Quantitation