Global mapping of CARM1 substrates


Collaborating Investigator/s

Wei Xu (University of Wisconsin-Madison)


Identification of CARM1 substrates in this project relied on the ability to confidently detect disappearance of methylation sites in CARM1 knockout cells, which could only be accomplished using highly reproducible multiplexed quantitative workflows. This project established novel methodology for direct global profiling of arginine methylation in vivo and refined enrichment and data generation practices necessary for effective analyses of this understudied PTM that could be easily reproduced in future studies.