09 Mar 2017

Reagents list

09 Mar 2017

TMT 10 Plex Labels

Isobaric labels that conjugate to primary amines. Good for multiplexed analysis of up to 10 samples.

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BCA Kit Protein Quantitation Kit

Colorometric protein quantitation kit. Good for knowing how much protein you have.

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Peptide Colorimetric Quantitation Kit

Colorometric peptide quantitation kit. Good for knowing how much peptide you have. We use this to make sure we inject the same amount of every sample for quantitative assays which is vital for LFQ

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Trypsin

Cleaves proteins C-terminal to lys or arg. A necessity for many proteomic applications. 

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Strata X Desalting Column

Quick easy gravity driven solid phase extraction cartidges.

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Titanium IMAC

Titatanium (IV) on a bead. We use this for rapid phosphorylation enrichment for phosphoproteomic analyses.

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BEH Column 1.7 µm C18

UPLC reversed phase material.  We use this to fabricate nano columns.  It has a good mix of high retention, stability, and separation efficiency. 

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BEH Column 5 µm C18

HPLC reversed phase material. We use this for bridging the tip of our fabricated nano columns.

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75/360 µm ID/OD Capillary

Provides structural walls and emitter tip of our fabricated columns.  We find these dimensions are the relatively easy to work with and provide the best results for us.

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