Viewing posts tagged neurodegeneration

Biologically relevant proteins in Alzheimer’s Disease

Proteomic analysis of cerebrospinal fluid (CSF) holds great promise in understanding the progression of neurodegenerative diseases, including Alzheimer’s disease (AD). As one of the primary reservoirs of neuronal biomolecules, CSF provides a window into the biochemical and cellular aspects of the neurological environment. Using mass spectrometry technologies, McKetney et. al. quantified 700 proteins across 10 pairs of age- and sex-matched participants. Using the paired structure, they identified a small group of biologically relevant proteins that show substantial changes in abundance between normal and AD participants. These findings suggest the utility of fractionating a single sample and using matching to increase proteomic depth in CSF.

Read the article: Pilot Proteomic Analysis of Cerebrospinal Fluid in Alzheimer’s Disease. Proteomics Clinical Applications.

McKetney Publishes “Proteomic Atlas of the Human Brain in Alzheimer’s Disease”

Justin McKetney et. al. recently published a paper on neurodegenerative diseases of the brain, such as Alzheimer’s disease (AD). Using state-of-the-art mass spectrometry, the group identified a core brain proteome where substantial differences were identified between previous proteomic studies of mature adult brains and their aged cohort. These findings suggest considerable value in examining specifically the brain proteome of aged human populations and can serve as a guide for how specific regions of the brain are affected by aging and neurodegeneration.

Graphical abstract for McKetney et al depicting areas of the brain studies, and methods used for creating a proteomic atlas: protein extraction, fractionation, chromatography, tandem MS, and Database search.