Viewing posts tagged glycans

Chemical labeling for glycan and glycopeptide quantitation

Growing implications of glycosylation in human disease have prompted intensive focus on revealing glycomic perturbations through absolute and relative quantification. Empowered by an increasing capacity for detection, identification, and characterization, the past decade has provided a significant increase in the number of suitable strategies for glycan and glycopeptide quantification. In this review, Delafield and Li present the most recent advances in chemical labeling and associated techniques for glycan and glycopeptide quantification.

Read the article: Recent Advances in Analytical Approaches for Glycan and Glycopeptide Quantitation

Collaboration explores the role of N-glycans during vertebrate development

A collaboration with the lab of Norman Dovichi at the University of Notre Dame explores the role of N-glycans in biological processes during vertebrate development. In a recent publication, Quantitative Capillary Zone Electrophoresis-Mass Spectrometry Reveals the N-glycome Developmental Plan during Vertebrate Embryogenesis, they report on the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in the African clawed frog. In the study, N-Glycans were labeled with isobaric tandem mass tags and characterized using tandem mass spectrometry. Over two thirds of the N-glycoproteins identified in the late stage were associated with neuron projection morphogenesis, suggesting a vital role of the N-glycome in neuronal development.