dimethyl leucine (DiLeu) isobaric labeling

Viewing posts tagged dimethyl leucine (DiLeu) isobaric labeling

Introducing DiLeuPMP, a multiplexed isobaric labeling method

Glycosylation plays an important role in how the human body functions, including cell recognition, signaling, and immune response. While efforts have been devoted to the analysis of N-glycans, high-throughput quantitative analysis of O-glycans is underexplored. In this study, a multiplexed isobaric labeling method, DiLeuPMP, is introduced. This method combines the release and labeling of O-glycans in one step and achieves accurate MS2-based relative quantification. This method provides an effective and reliable approach for the profiling and high-throughput quantitative analysis of O-glycans in complex samples.

Read the article: DiLeuPMP: a multiplexed isobaric labeling method for quantitative analysis of O-glycans

Li lab project uses DiLeu isobaric labeling to monitor neurotoxicity in chemotherapy in children

A new project by the Lingjun Li lab explores the use of the 4-plex N,N-dimethyl leucine (DiLeu) isobaric labeling strategy for protein identification and quantification in cerebrospinal fluid in children undergoing chemotherapy. The results from this and future studies will provide a means to monitor neurotoxicity and develop strategies to prevent central nervous system injury in response to chemotherapy in children.

The publication is Isobaric labeling strategy utilizing 4-plex N,N-dimethyl leucine (DiLeu) tags reveals proteomic changes induced by chemotherapy in cerebrospinal fluid of children with B-cell acute lymphoblastic leukemia from the Journal of Proteome Research.