DiLeu

Viewing posts tagged DiLeu

07 Oct 2021

DiLeu tagging provides insight into human pancreas development

By nickwiecien

The extracellular matrix (ECM) is unique to each tissue, it guides cell differentiation, migration, morphology, and function. It has not been systematically studied in the human pancreas. In this paper, Li et al describe how they used mass spectrometry-based strategies using N,N-dimethyl leucine isobaric tags to identify proteome-wide and ECM-specific alterations in four age groups. They found 3,523 proteins and quantified 117 of them. This work will contribute to understanding the critical roles ECM plays in human pancreas development and maturation.

Read the article: Proteome-wide and matrisome-specific alterations during human pancreas development and maturation

08 Sep 2021

Introducing DiLeuPMP, a multiplexed isobaric labeling method

By nickwiecien

Glycosylation plays an important role in how the human body functions, including cell recognition, signaling, and immune response. While efforts have been devoted to the analysis of N-glycans, high-throughput quantitative analysis of O-glycans is underexplored. In this study, a multiplexed isobaric labeling method, DiLeuPMP, is introduced. This method combines the release and labeling of O-glycans in one step and achieves accurate MS2-based relative quantification. This method provides an effective and reliable approach for the profiling and high-throughput quantitative analysis of O-glycans in complex samples.

Read the article: DiLeuPMP: a multiplexed isobaric labeling method for quantitative analysis of O-glycans

02 Dec 2020

Gut microbiome may play a role in brain functions and behaviors

By nickwiecien

Gut microbiota can regulate host physiological and pathological status through gut–brain communications or pathways. However, the impact of the gut microbiome on the proteins involved in regulating brain functions and behaviors is still not clearly understood. In a recent publication by Liu et al., the author describes a combined label-free and 10-plex DiLeu-based quantitative method that enabled a comprehensive profiling of gut microbiome that induced dynamic changes, suggesting that the gut microbiome might mediate a range of behavioral changes, brain development, and learning and memory through these neuropeptides and proteins.

Liu R et al. Integrated Label-Free and 10-plex DiLeu Isobaric Tag Quantitative Methods for Profiling Changes in the Mouse Hypothalamic Neuropeptidome and Proteome: Assessment of the Impact of the Gut Microbiome. Analytical Chemistry.

04 Nov 2020

DiLeu tagging for protein and phosphorylation quantification in parallel

By nickwiecien

For the first time, DiLeu tagging has been implemented for protein and phosphorylation quantification in parallel. This process was described in a recent publication by Zhong et al titled Highly multiplexed quantitative proteomic and phosphoproteomic analyses in vascular smooth muscle cell dedifferentiation.

The research team developed a strategy that used 12-plex N,N-dimethyl leucine (DiLeu) isobaric tags together with the DiLeu software tool to globally assess protein expression and phosphorylation changes in smooth muscle cells (SMCs) treated with TGFβ/Smad3 and/or SDF-1α (stromal cell-derived factor).

The goal of this work was to develop insights into the mechanisms of TGFβ regulated SMC dedifferentiation, as well as effective therapeutics for vascular disease.