topic

Rapid preparation of human blood plasma for bottom-up proteomics analysis

description


This protocol offers step-by-step instructions for preparation of raw blood plasma for liquid chromatography – tandem mass spectrometry (LC-MS/MS) analysis in clinical proteomics studies. The entire transformation from plasma proteins to desalted tryptic peptides takes only 3–4 h. The protocol can be adopted for large-scale studies and automation.