It seems like with every new generation of mass spectrometer the optimal methods and settings change. We have considerable experience with all models of Orbitrap and Orbitrap hybrid systems and can offer training on their most efficient implementation.
Knowing when your system is underperforming is essential. We have developed methods and procedures for monitoring instrument performance. We are always happy to share our strategies for this critical, yet rarely discussed, topic.
Peptide separations are critical to proteomic success. No matter your expertise level nanochromatography is likely a challenge. This technique, in our opinion, is often overlooked as the limiting factor in obtaining great results. We deal with these issues daily. If you are struggling with separations, or just want to learn how we make and use columns, please let us know.