Shotgun proteomics relies on the analysis and detection of peptides – each ~6 to 20 amino acids in length – created following enzymatic proteolysis of intact proteins. Incomplete and/or semi-specific digestion can really dampen your results. If you are having issues with digestion we can assist by sharing with you our protocols and helping you assess the quality of your digestion.
- trypsin
- chymotrypsin
- LysC
- GluC
- AspN
- LysN